Ayahuasca (a compound obtained from the combination of two plants) can be considered as an artifact, and more precisely as a medial one. Ayahuasca has its roots in Amazonian indigenous cultures, where it is used in shamanic rituals, it’s believed to have healing powers, and to trigger a spiritual awareness of transcendence. There is a mediating function of the artifact, typical of religious practices, which in this case enacts a particular experience with visual hallucinations, disphoric and euphoric features. Recent neurological researches show that Ayahuasca interacts with serotonin receptors, and that this is the cause of the consciousness’ alteration. This experience receives a spiritual interpretation in the Amazonian rituals, but it’s alsoan object of attention for medical researchers especially in relation to psychiatric issues. Nowadays the use of Ayahuasca has spread worldwide, in some cases as an attempt to reproduce the original ritual, in other as a way to explore an altered state of consciousness, similar to the hippie’s culture, eventually recording and sharing the experience on the internet.These different contexts approach Ayahuasca’s agency differently, but they are all efforts to explore a sort of black-box of human-nature interactions, where by black-box we refer to something that produces effects, but whose operational logic is unknown. This metaphor is also used to describe recent AI technologies, which sometimes can generate hallucinations, whose emergence is not always known. As in the case of hallucinations induced by Ayahuasca, their interpretations change in different discursive fields, and semiotic can help understanding this changes.