The paper highlights the lingua-bio-semiotic methodology of analyzing emotions in narrative discourse. It addresses the necessity of underrepresented research on the lingo-semiotic nature of subjectivity as an emergent phenomenon. Thus, the paper disclosed the semiotic essenceof subjectivity in the narrative discourse and articulated adequate means and ways of its materialization. Applying the methodology of narrative poetics, the definition of cognitive levels of knowledge acquisition by cognitive linguistics, and recognition of the dialogical nature of the personality in analytical philosophy, an autopoietic model of emotions in narrative discourse has been suggested. The results of the research have prompted the efficiency of the analysis of emotions from the biosemiotic and cultural approaches. The integrated approach unites the application of the methods of the modelling system with the complex sign systems, including narrative discourse. The key agency in the meaning creation in the intersubjective interaction is ascribed to the subject-observer. The model of the subject-observer is represented in a form of double interpretation. On the one hand, it incorporates the relation of the sign vehicle and the representamen, and, on the other, its relation to the sign itself outside the level of interpretation. The dynamic character of the subject as a certain type of narrative construct has been observed, which emerges in the process of narrative unfolding, counts on the “languaging” (in H. Maturana’s terms), and holistic or discrete quality of the object.